› 2jev

X-ray diffraction
2.3Å resolution

Crystal structure of human spermine,spermidine acetyltransferase in complex with a bisubstrate analog (N1-acetylspermine-S-CoA).

Released:
DOI: 10.2210/pdb2jev/pdb
PDB entry 2jev coloured by chain, front view.
PDB entry 2jev coloured by chain, side view.
PDB entry 2jev coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Mechanistic and structural analysis of human spermidine/spermine N1-acetyltransferase.
Hegde SS, Chandler J, Vetting MW, Yu M, Blanchard JS
Biochemistry 46 7187-95 (2007)
PMID: 17516632

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149349 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diamine acetyltransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 174 amino acids
Theoretical weight: 20.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21673 (Residues: 1-171; Coverage: 100%)
Gene names: SAT, SAT1
Sequence domains: Acetyltransferase (GNAT) family
Structure domains: Aminopeptidase

Ligands and Environments


Cofactor: Ligand NHQ 2 x NHQ
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43
Unit cell:
a: 46.729Å b: 46.729Å c: 191.792Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.224 0.275
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Polyamine catabolism and disease.
Casero et al. (2009)
3 more

6 mentions without citation

Structure and Functional Diversity of GCN5-Related N-Acetyltransferases (GNAT).
Salah Ud-Din et al. (2016)
5 more