› 2jf1

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE FILAMIN A REPEAT 21 COMPLEXED WITH THE INTEGRIN BETA2 CYTOPLASMIC TAIL PEPTIDE

Released:
DOI: 10.2210/pdb2jf1/pdb
PDB entry 2jf1 coloured by chain, front view.
PDB entry 2jf1 coloured by chain, side view.
PDB entry 2jf1 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Beta2 integrin phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding.
Takala H, Nurminen E, Nurmi SM, Aatonen M, Strandin T, Takatalo M, Kiema T, Gahmberg CG, Ylänne J, Fagerholm SC
Blood 112 1853-62 (2008)
PMID: 18550856

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138501 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Filamin-A Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 9.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P21333 (Residues: 2236-2329; Coverage: 4%)
Gene names: FLN, FLN1, FLNA
Sequence domains: Filamin/ABP280 repeat
Structure domains: Immunoglobulins
Integrin beta-2 Chain: T
Molecule details ›
Chain: T
Length: 35 amino acids
Theoretical weight: 4.21 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P05107 (Residues: 735-769; Coverage: 5%)
Gene names: CD18, ITGB2, MFI7
Sequence domains: Integrin beta cytoplasmic domain

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P213
Unit cell:
a: 78.72Å b: 78.72Å c: 78.72Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.247 0.278
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

33 review citations

Integrin signalling at a glance.
Harburger et al. (2009)
32 more

3 mentions without citation

Cystic fibrosis transmembrane conductance regulator interacts with multiple immunoglobulin domains of filamin A.
Playford et al. (2010)
2 more