› 2jga

X-ray diffraction
3.01Å resolution

Crystal structure of human cytosolic 5'-nucleotidase III in complex with phosphate and magnesium

Released:
DOI: 10.2210/pdb2jga/pdb
PDB entry 2jga coloured by chain, front view.
PDB entry 2jga coloured by chain, side view.
PDB entry 2jga coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition.
Walldén K, Stenmark P, Nyman T, Flodin S, Gräslund S, Loppnau P, Bianchi V, Nordlund P
J Biol Chem 282 17828-36 (2007)
PMID: 17405878

Function and Biology Details

Reactions catalysed: 
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
CMP + H(2)O = cytidine + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic 5'-nucleotidase 3A Chain: A
Molecule details ›
Chain: A
Length: 292 amino acids
Theoretical weight: 33.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H0P0 (Residues: 61-336; Coverage: 82%)
Gene names: HSPC233, NT5C3, NT5C3A, P5N1, UMPH1
Sequence domains: Pyrimidine 5'-nucleotidase (UMPH-1)
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 87.459Å b: 101.036Å c: 77.046Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.181 0.268
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Therapeutic potentials of ecto-nucleoside triphosphate diphosphohydrolase, ecto-nucleotide pyrophosphatase/phosphodiesterase, ecto-5'-nucleotidase, and alkaline phosphatase inhibitors.
al-Rashida et al. (2014)
4 more

1 mention without citation

Cytosolic 5'-nucleotidase III (NT5C3): gene sequence variation and functional genomics.
Aksoy et al. (2009)