› 2jht

X-ray diffraction
1.88Å resolution

CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT

Released:
DOI: 10.2210/pdb2jht/pdb
PDB entry 2jht coloured by chain, front view.
PDB entry 2jht coloured by chain, side view.
PDB entry 2jht coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Protein crystallization by surface entropy reduction: optimization of the SER strategy.
Cooper DR, Boczek T, Grelewska K, Pinkowska M, Sikorska M, Zawadzki M, Derewenda Z
Acta Crystallogr D Biol Crystallogr 63 636-45 (2007)
PMID: 17452789

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156565 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GDP-dissociation inhibitor 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 138 amino acids
Theoretical weight: 15.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P52565 (Residues: 67-202; Coverage: 67%)
Gene names: ARHGDIA, GDIA1
Sequence domains: RHO protein GDP dissociation inhibitor
Structure domains: Coagulation Factor XIII, subunit A, domain 1

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand LI 1 x LI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2
Unit cell:
a: 190.832Å b: 34.022Å c: 103.94Å
α: 90° β: 115.59° γ: 90°
R-values:
R R work R free
0.223 0.222 0.259
Expression system: Escherichia coli BL21(DE3)

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Citations

15 review citations

The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.
Koide et al. (2009)
14 more