› 2jif

X-ray diffraction
2Å resolution

Structure of human short-branched chain acyl-CoA dehydrogenase (ACADSB)

Released:
DOI: 10.2210/pdb2jif/pdb
PDB entry 2jif coloured by chain, front view.
PDB entry 2jif coloured by chain, side view.
PDB entry 2jif coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Pike ACW, Hozjan V, Smee C, Niesen FH, Kavanagh KL, Umeano C, Turnbull AP, von Delft F, Weigelt J, Edwards A, Arrowsmith CH, Sundstrom M, Oppermann U

Function and Biology Details

Reaction catalysed: 
2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H(+)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155370 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 404 amino acids
Theoretical weight: 44.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P45954 (Residues: 51-432; Coverage: 88%)
Gene name: ACADSB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
3 bound ligands:
Ligand COS 4 x COS
Ligand EDO 7 x EDO
Ligand CL 10 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 150.245Å b: 150.245Å c: 201.586Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.184 0.209
Expression system: Escherichia coli BL21(DE3)

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