› 2jii

X-ray diffraction
2Å resolution

Structure of vaccinia related kinase 3

Released:
DOI: 10.2210/pdb2jii/pdb
PDB entry 2jii coloured by chain, front view.
PDB entry 2jii coloured by chain, side view.
PDB entry 2jii coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the pseudokinase VRK3 reveals a degraded catalytic site, a highly conserved kinase fold, and a putative regulatory binding site.
Scheeff ED, Eswaran J, Bunkoczi G, Knapp S, Manning G
Structure 17 128-38 (2009)
PMID: 19141289

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase VRK3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 352 amino acids
Theoretical weight: 39.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IV63 (Residues: 138-474; Coverage: 71%)
Gene name: VRK3
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 91.258Å b: 54.423Å c: 91.822Å
α: 90° β: 91.22° γ: 90°
R-values:
R R work R free
0.181 0.179 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

37 review citations

Protein kinases: evolution of dynamic regulatory proteins.
Taylor et al. (2011)
36 more

10 mentions without citation

Pseudokinases-remnants of evolution or key allosteric regulators?
Zeqiraj et al. (2010)
9 more