› 2jis

X-ray diffraction
1.6Å resolution

Human cysteine sulfinic acid decarboxylase (CSAD) in complex with PLP.

Released:
DOI: 10.2210/pdb2jis/pdb
PDB entry 2jis coloured by chain, front view.
PDB entry 2jis coloured by chain, side view.
PDB entry 2jis coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Collins R, Moche M, Arrowsmith C, Berglund H, Busam R, Dahlgren LG, Edwards A, Flodin S, Flores A, Graslund S, Hammarstrom M, Hallberg BM, Johansson I, Kallas A, Karlberg T, Kotenyova T, Lehtio L, Nordlund P, Nyman T, Ogg D, Persson C, Sagemark J, Stenmark P, Sundstrom M, Thorsell AG, Tresaugues L, van den Berg S, Weigelt J, Welin M, Holmberg-Schiavone L, Structural Genomics Consortium (SGC)

Function and Biology Details

Reactions catalysed: 
L-aspartate = beta-alanine + CO(2)
3-sulfino-L-alanine = hypotaurine + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195322 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine sulfinic acid decarboxylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 515 amino acids
Theoretical weight: 57.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y600 (Residues: 1-493; Coverage: 100%)
Gene names: CSAD, CSD
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
1 bound ligand:
Ligand NO3 2 x NO3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 65.318Å b: 100.636Å c: 163.104Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.154 0.153 0.179
Expression system: Escherichia coli BL21(DE3)

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