› 2jjs

X-ray diffraction
1.85Å resolution

Structure of human CD47 in complex with human signal regulatory protein (SIRP) alpha

Released:
DOI: 10.2210/pdb2jjs/pdb
PDB entry 2jjs coloured by chain, front view.
PDB entry 2jjs coloured by chain, side view.
PDB entry 2jjs coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.
Hatherley D, Graham SC, Turner J, Harlos K, Stuart DI, Barclay AN
Mol Cell 31 266-77 (2008)
PMID: 18657508

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160234 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type substrate 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 126 amino acids
Theoretical weight: 13.92 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P78324 (Residues: 31-149; Coverage: 25%)
Gene names: BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP, SIRPA
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Leukocyte surface antigen CD47 Chains: C, D
Molecule details ›
Chains: C, D
Length: 127 amino acids
Theoretical weight: 14.52 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: Q08722 (Residues: 19-136; Coverage: 39%)
Gene names: CD47, MER6
Sequence domains: CD47 immunoglobulin-like domain
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
Ligand NAG 6 x NAG
Ligand IOD 18 x IOD
1 modified residue:
Ligand PCA 2 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2, ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 42.477Å b: 72.41Å c: 89.826Å
α: 90° β: 95.01° γ: 90°
R-values:
R R work R free
0.171 0.169 0.213
Expression system: Cricetulus griseus

Quick links

Citations

35 review citations

The interaction between signal regulatory protein alpha (SIRPĪ±) and CD47: structure, function, and therapeutic target.
Barclay et al. (2014)
34 more

32 mentions without citation

Peptide and protein nanoparticle conjugates: versatile platforms for biomedical applications.
Spicer et al. (2018)
31 more