› 2jkt

X-ray diffraction
3.4Å resolution

AP2 CLATHRIN ADAPTOR CORE with CD4 Dileucine peptide RM(phosphoS) EIKRLLSE Q to E mutant

Released:
DOI: 10.2210/pdb2jkt/pdb
PDB entry 2jkt coloured by chain, front view.
PDB entry 2jkt coloured by chain, side view.
PDB entry 2jkt coloured by chain, top view.
Source organisms:
Primary publication:
A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex.
Kelly BT, McCoy AJ, Späte K, Miller SE, Evans PR, Höning S, Owen DJ
Nature 456 976-979 (2008)
PMID: 19140243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134530 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
AP-2 complex subunit alpha-2 Chains: A, L
Molecule details ›
Chains: A, L
Length: 623 amino acids
Theoretical weight: 69.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17427 (Residues: 1-271, 272-622; Coverage: 66%)
Gene names: Adtab, Ap2a2
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit beta Chains: B, E
Molecule details ›
Chains: B, E
Length: 591 amino acids
Theoretical weight: 66.95 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63010 (Residues: 1-591; Coverage: 63%)
Gene names: ADTB2, AP2B1, CLAPB1
Sequence domains: Adaptin N terminal region
Structure domains: Leucine-rich Repeat Variant
AP-2 complex subunit sigma Chains: I, S
Molecule details ›
Chains: I, S
Length: 142 amino acids
Theoretical weight: 17.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62743 (Residues: 1-142; Coverage: 100%)
Gene names: Ap17, Ap2s1, Claps2
Sequence domains: Clathrin adaptor complex small chain
Structure domains: Beta-Lactamase
AP-2 complex subunit mu Chains: M, U
Molecule details ›
Chains: M, U
Length: 435 amino acids
Theoretical weight: 49.73 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P84092 (Residues: 1-435; Coverage: 100%)
Gene name: Ap2m1
Sequence domains:
Structure domains:
T-cell surface glycoprotein CD4 Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 11 amino acids
Theoretical weight: 1.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01730 (Residues: 431-441; Coverage: 3%)
Gene name: CD4
Sequence domains: T cell CD4 receptor C terminal region

Ligands and Environments

1 bound ligand:
Ligand SO4 31 x SO4
1 modified residue:
Ligand SEP 2 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P43212
Unit cell:
a: 171.2Å b: 171.2Å c: 324.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.202 0.256
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

61 review citations

Molecular mechanism and physiological functions of clathrin-mediated endocytosis.
McMahon et al. (2011)
60 more

3 mentions without citation

A structural explanation for the binding of endocytic dileucine motifs by the AP2 complex.
Kelly et al. (2008)
2 more