› 2jti

Solution NMR

Solution structure of the yeast iso-1-cytochrome c (T12A) : yeast cytochrome c peroxidase complex

Released:
DOI: 10.2210/pdb2jti/pdb
PDB entry 2jti coloured by chain, ensemble of 10 models, front view.
PDB entry 2jti coloured by chain, ensemble of 10 models, side view.
PDB entry 2jti coloured by chain, ensemble of 10 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Shifting the equilibrium between the encounter state and the specific form of a protein complex by interfacial point mutations.
Volkov AN, Bashir Q, Worrall JA, Ullmann GM, Ubbink M
J Am Chem Soc 132 11487-95 (2010)
PMID: 20672804

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132231 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.53 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00431 (Residues: 68-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:
Cytochrome c isoform 1 Chain: B
Molecule details ›
Chain: B
Length: 108 amino acids
Theoretical weight: 12.04 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00044 (Residues: 2-109; Coverage: 99%)
Gene names: CYC1, J1653, YJR048W
Sequence domains: Cytochrome c
Structure domains: Cytochrome c-like domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM

Cofactor: Ligand HEC 1 x HEC
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Dynamics in electron transfer protein complexes.
Bashir et al. (2011)
6 more