› 2jun

Solution NMR

Structure of the MID1 tandem B-boxes reveals an interaction reminiscent of intermolecular RING heterodimers

Released:
DOI: 10.2210/pdb2jun/pdb
PDB entry 2jun coloured by chain, ensemble of 20 models, front view.
PDB entry 2jun coloured by chain, ensemble of 20 models, side view.
PDB entry 2jun coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MID1 tandem B-boxes reveals an interaction reminiscent of intermolecular ring heterodimers.
Tao H, Simmons BN, Singireddy S, Jakkidi M, Short KM, Cox TC, Massiah MA
Biochemistry 47 2450-7 (2008)
PMID: 18220417

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Midline-1 Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O15344 (Residues: 114-214; Coverage: 15%)
Gene names: FXY, MID1, RNF59, TRIM18, XPRF
Sequence domains: B-box zinc finger
Structure domains: Classic Zinc Finger

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

RING domain E3 ubiquitin ligases.
Deshaies et al. (2009)
7 more

7 mentions without citation

14-3-3 scaffold proteins mediate the inactivation of trim25 and inhibition of the type I interferon response by herpesvirus deconjugases.
Gupta et al. (2019)
6 more