› 2k42

Solution NMR

Solution Structure of the GTPase Binding Domain of WASP in Complex with EspFU, an EHEC Effector

Released:
DOI: 10.2210/pdb2k42/pdb
PDB entry 2k42 coloured by chain, ensemble of 20 models, front view.
PDB entry 2k42 coloured by chain, ensemble of 20 models, side view.
PDB entry 2k42 coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Structural mechanism of WASP activation by the enterohaemorrhagic E. coli effector EspF(U).
Cheng HC, Skehan BM, Campellone KG, Leong JM, Rosen MK
Nature 454 1009-13 (2008)
PMID: 18650809

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154864 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin nucleation-promoting factor WAS Chain: A
Molecule details ›
Chain: A
Length: 72 amino acids
Theoretical weight: 8.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42768 (Residues: 242-310; Coverage: 14%)
Gene names: IMD2, WAS
Sequence domains: P21-Rho-binding domain
Structure domains: CRIB domain
ESPFU Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.07 KDa
Source organism: Escherichia coli O157:H7
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

23 review citations

Regulation of the actin cytoskeleton-plasma membrane interplay by phosphoinositides.
Saarikangas et al. (2010)
22 more

3 mentions without citation

Synthetic Protein Scaffolds Based on Peptide Motifs and Cognate Adaptor Domains for Improving Metabolic Productivity.
Horn et al. (2015)
2 more