› 2k7l

Solution NMR

NMR structure of a complex formed by the C-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1

Released:
DOI: 10.2210/pdb2k7l/pdb
PDB entry 2k7l coloured by chain, ensemble of 21 models, front view.
PDB entry 2k7l coloured by chain, ensemble of 21 models, side view.
PDB entry 2k7l coloured by chain, ensemble of 21 models, top view.
Source organism: Homo sapiens
Primary publication:
NMR structure of a complex formed by the carboxyl-terminal domain of human RAP74 and a phosphorylated peptide from the central domain of the FCP1 phosphatase.
Yang A, Abbott KL, Desjardins A, Di Lello P, Omichinski JG, Legault P
Biochemistry 48 1964-74 (2009)
PMID: 19215094

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152878 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
RNA polymerase II subunit A C-terminal domain phosphatase Chain: B
Molecule details ›
Chain: B
Length: 19 amino acids
Theoretical weight: 2.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9Y5B0 (Residues: 582-600; Coverage: 2%)
Gene names: CTDP1, FCP1
General transcription factor IIF subunit 1 Chain: A
Molecule details ›
Chain: A
Length: 67 amino acids
Theoretical weight: 7.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35269 (Residues: 451-517; Coverage: 13%)
Gene names: GTF2F1, RAP74
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 71%
Refinement method: torsion angle dynamics
Expression systems:
  • Not provided
  • Escherichia coli

Quick links

Citations

4 review citations

Structural basis of transcription initiation by RNA polymerase II.
Sainsbury et al. (2015)
3 more

1 mention without citation

Regulation of Androgen Receptor Activity by Transient Interactions of Its Transactivation Domain with General Transcription Regulators.
De Mol et al. (2018)