› 2kbt

Solution NMR

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Released:
DOI: 10.2210/pdb2kbt/pdb
PDB entry 2kbt coloured by chain, ensemble of 20 models, front view.
PDB entry 2kbt coloured by chain, ensemble of 20 models, side view.
PDB entry 2kbt coloured by chain, ensemble of 20 models, top view.
Source organisms:
Primary publication:
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method.
Kobashigawa Y, Kumeta H, Ogura K, Inagaki F
J Biomol NMR 43 145-50 (2009)
PMID: 19140010

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G; Proto-oncogene vav Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.97 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P27870 (Residues: 784-844; Coverage: 7%)
  • Canonical: P06654 (Residues: 228-282; Coverage: 13%)
Gene names: Vav, Vav1, spg
Sequence domains:
Structure domains: SH3 Domains

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 91%
Refinement method: simulated annealing, distance geometry
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Making and breaking peptide bonds: protein engineering using sortase.
Popp et al. (2011)
15 more

4 mentions without citation

Overcoming the solubility limit with solubility-enhancement tags: successful applications in biomolecular NMR studies.
Zhou et al. (2010)
3 more