› 2kem

Solution NMR

Extended structure of citidine deaminase domain of APOBEC3G

Released:
DOI: 10.2210/pdb2kem/pdb
PDB entry 2kem coloured by chain, ensemble of 10 models, front view.
PDB entry 2kem coloured by chain, ensemble of 10 models, side view.
PDB entry 2kem coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
An extended structure of the APOBEC3G catalytic domain suggests a unique holoenzyme model.
Harjes E, Gross PJ, Chen KM, Lu Y, Shindo K, Nowarski R, Gross JD, Kotler M, Harris RS, Matsuo H
J Mol Biol 389 819-32 (2009)
PMID: 19389408

Function and Biology Details

Reaction catalysed: 
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA dC->dU-editing enzyme APOBEC-3G Chain: A
Molecule details ›
Chain: A
Length: 202 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HC16 (Residues: 191-384; Coverage: 51%)
Gene names: APOBEC3G, MDS019
Sequence domains: APOBEC-like C-terminal domain

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Interactions of host APOBEC3 restriction factors with HIV-1 in vivo: implications for therapeutics.
Albin et al. (2010)
18 more

13 mentions without citation

Suppression of APOBEC3-mediated restriction of HIV-1 by Vif.
Feng et al. (2014)
12 more