› 2khu

Solution NMR

Solution Structure of the Ubiquitin-Binding Motif of Human Polymerase Iota

Released:
DOI: 10.2210/pdb2khu/pdb
PDB entry 2khu coloured by chain, ensemble of 25 models, front view.
PDB entry 2khu coloured by chain, ensemble of 25 models, side view.
PDB entry 2khu coloured by chain, ensemble of 25 models, top view.
Source organisms:
Primary publication:
Unconventional ubiquitin recognition by the ubiquitin-binding motif within the Y family DNA polymerases iota and Rev1.
Bomar MG, D'Souza S, Bienko M, Dikic I, Walker GC, Zhou P
Mol Cell 37 408-17 (2010)
PMID: 20159559

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148790 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase iota; Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 12.35 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P19909 (Residues: 304-359; Coverage: 10%)
  • Canonical: Q9UNA4 (Residues: 699-740; Coverage: 6%)
Gene names: POLI, RAD30B, spg
Sequence domains: B domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Ubiquitin-binding proteins: decoders of ubiquitin-mediated cellular functions.
Husnjak et al. (2012)
19 more

4 mentions without citation

An overview of Y-Family DNA polymerases and a case study of human DNA polymerase η.
Yang W. (2014)
3 more