› 2kkf

Solution NMR

Solution structure of MLL CXXC domain in complex with palindromic CPG DNA

Released:
DOI: 10.2210/pdb2kkf/pdb
PDB entry 2kkf coloured by chain, ensemble of 10 models, front view.
PDB entry 2kkf coloured by chain, ensemble of 10 models, side view.
PDB entry 2kkf coloured by chain, ensemble of 10 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the MLL CXXC domain-DNA complex and its functional role in MLL-AF9 leukemia.
Cierpicki T, Risner LE, Grembecka J, Lukasik SM, Popovic R, Omonkowska M, Shultis DD, Zeleznik-Le NJ, Bushweller JH
Nat Struct Mol Biol 17 62-8 (2010)
PMID: 20010842

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115487 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
MLL cleavage product N320 Chain: A
Molecule details ›
Chain: A
Length: 59 amino acids
Theoretical weight: 6.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 1147-1203; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
Sequence domains: CXXC zinc finger domain
5'-D(*CP*CP*CP*TP*GP*CP*GP*CP*AP*GP*GP*G)-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: SIMULATED ANNEALING, DISTANCE GEOMETRY
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

31 review citations

Role of TET enzymes in DNA methylation, development, and cancer.
Rasmussen et al. (2016)
30 more

12 mentions without citation

ZF-CxxC domain-containing proteins, CpG islands and the chromatin connection.
Long et al. (2013)
11 more