› 2kn7

Solution NMR

Structure of the XPF-single strand DNA complex

Released:
DOI: 10.2210/pdb2kn7/pdb
PDB entry 2kn7 coloured by chain, ensemble of 20 models, front view.
PDB entry 2kn7 coloured by chain, ensemble of 20 models, side view.
PDB entry 2kn7 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The structure of the XPF-ssDNA complex underscores the distinct roles of the XPF and ERCC1 helix- hairpin-helix domains in ss/ds DNA recognition.
Das D, Folkers GE, van Dijk M, Jaspers NG, Hoeijmakers JH, Kaptein R, Boelens R
Structure 20 667-75 (2012)
PMID: 22483113

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115491 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA repair endonuclease XPF Chains: A, D
Molecule details ›
Chains: A, D
Length: 67 amino acids
Theoretical weight: 7.42 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92889 (Residues: 842-908; Coverage: 7%)
Gene names: ERCC11, ERCC4, XPF
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
DNA (5'-D(*CP*AP*GP*TP*GP*GP*CP*TP*GP*A)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

DNA repair endonuclease ERCC1-XPF as a novel therapeutic target to overcome chemoresistance in cancer therapy.
McNeil et al. (2012)
5 more

5 mentions without citation

XPA: A key scaffold for human nucleotide excision repair.
Sugitani et al. (2016)
4 more