› 2kpz

Solution NMR

Human NEDD4 3RD WW Domain Complex with The Human T-cell Leukemia virus 1 GAG-Pro poliprotein Derived Peptide SDPQIPPPYVEP

Released:
DOI: 10.2210/pdb2kpz/pdb
PDB entry 2kpz coloured by chain, ensemble of 20 models, front view.
PDB entry 2kpz coloured by chain, ensemble of 20 models, side view.
PDB entry 2kpz coloured by chain, ensemble of 20 models, top view.
Source organisms:
Entry authors: Iglesias-Bexiga M, Macias M, Bonet R, Blanco FJ, Cobos ES, Luque I

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142985 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4 Chain: A
Molecule details ›
Chain: A
Length: 49 amino acids
Theoretical weight: 5.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46934 (Residues: 834-878; Coverage: 3%)
Gene names: KIAA0093, NEDD4, NEDD4-1, PIG53, RPF1
Sequence domains: WW domain
Matrix protein p19 Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.34 KDa
Source organism: HTLV-1 isolate Mel 15
Expression system: Not provided
UniProt:
  • Canonical: P0C210 (Residues: 113-124; Coverage: 2%)
Gene names: gag-pro, prt

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 73%
Refinement method: DGSA-distance geometry simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links