› 2krj

Solid-state NMR

High-Resolution Solid-State NMR Structure of a 17.6 kDa Protein

Released:
DOI: 10.2210/pdb2krj/pdb
PDB entry 2krj coloured by chain, ensemble of 20 models, front view.
PDB entry 2krj coloured by chain, ensemble of 20 models, side view.
PDB entry 2krj coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution solid-state NMR structure of a 17.6 kDa protein.
Bertini I, Bhaumik A, De Paëpe G, Griffin RG, Lelli M, Lewandowski JR, Luchinat C
J Am Chem Soc 132 1032-40 (2010)
PMID: 20041641

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 16.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 112-263; Coverage: 34%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

1 bound ligand:
Ligand CO 1 x CO
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

11 review citations

Protein structure determination by magic-angle spinning solid-state NMR, and insights into the formation, structure, and stability of amyloid fibrils.
Comellas et al. (2013)
10 more

3 mentions without citation

High-resolution solid-state NMR structure of a 17.6 kDa protein.
Bertini et al. (2010)
2 more