› 2kyg

Solution NMR

Structure of the AML1-ETO Nervy Domain - PKA(RIIa) complex and its contribution to AML1-ETO activity

Released:
DOI: 10.2210/pdb2kyg/pdb
PDB entry 2kyg coloured by chain, ensemble of 15 models, front view.
PDB entry 2kyg coloured by chain, ensemble of 15 models, side view.
PDB entry 2kyg coloured by chain, ensemble of 15 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the AML1-ETO NHR3-PKA(RIIα) complex and its contribution to AML1-ETO activity.
Corpora T, Roudaia L, Oo ZM, Chen W, Manuylova E, Cai X, Chen MJ, Cierpicki T, Speck NA, Bushweller JH
J Mol Biol 402 560-77 (2010)
PMID: 20708017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146800 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase type II-alpha regulatory subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 50 amino acids
Theoretical weight: 5.62 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13861 (Residues: 1-45; Coverage: 11%)
Gene names: PKR2, PRKAR2, PRKAR2A
Sequence domains: Regulatory subunit of type II PKA R-subunit
Structure domains: cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain
Protein CBFA2T1 Chain: C
Molecule details ›
Chain: C
Length: 38 amino acids
Theoretical weight: 4.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06455 (Residues: 437-467; Coverage: 5%)
Gene names: AML1T1, CBFA2T1, CDR, ETO, MTG8, RUNX1T1, ZMYND2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 92%
Refinement method: simulated annealing
Expression system: Escherichia coli

Quick links

Citations

4 review citations

RUNX1 and RUNX1-ETO: roles in hematopoiesis and leukemogenesis.
Lam et al. (2012)
3 more

3 mentions without citation

Structure of the AML1-ETO NHR3-PKA(RIIα) complex and its contribution to AML1-ETO activity.
Corpora et al. (2010)
2 more