› 2l05

Solution NMR

Solution NMR Structure of the Ras-binding domain of Serine/threonine-protein kinase B-raf from Homo sapiens, Northeast Structural Genomics Consortium Target HR4694F

Released:
DOI: 10.2210/pdb2l05/pdb
PDB entry 2l05 coloured by chain, ensemble of 20 models, front view.
PDB entry 2l05 coloured by chain, ensemble of 20 models, side view.
PDB entry 2l05 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Aramini JM, Janjua H, Ciccosanti C, Shastry R, Huang YJ, Acton TB, Xiao R, Everett JK, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147194 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein kinase B-raf Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15056 (Residues: 149-232; Coverage: 11%)
Gene names: BRAF, BRAF1, RAFB1
Sequence domains: Raf-like Ras-binding domain
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 93%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

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