› 2l0b

Solution NMR

Solution NMR structure of zinc finger domain of E3 ubiquitin-protein ligase praja-1 from Homo sapiens, Northeast Structural Genomics Consortium (NESG) target HR4710B

Released:
DOI: 10.2210/pdb2l0b/pdb
PDB entry 2l0b coloured by chain, ensemble of 20 models, front view.
PDB entry 2l0b coloured by chain, ensemble of 20 models, side view.
PDB entry 2l0b coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Liu G, Tong S, Hamilton K, Ciccosanti C, Shastry R, Acton TB, Xiao R, Everett JK, Montelione GT, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185542 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Praja-1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 10.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NG27 (Residues: 564-643; Coverage: 12%)
Gene names: PJA1, RNF70
Sequence domains: Ring finger domain
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry, simulated annealing, molecular dynamics
Expression system: Escherichia coli

Quick links