› 2l0i

Solution NMR

Solution structure of Rtt103 CTD-interacting domain bound to a Ser2 phosphorylated CTD peptide

Released:
DOI: 10.2210/pdb2l0i/pdb
PDB entry 2l0i coloured by chain, ensemble of 10 models, front view.
PDB entry 2l0i coloured by chain, ensemble of 10 models, side view.
PDB entry 2l0i coloured by chain, ensemble of 10 models, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain.
Lunde BM, Reichow SL, Kim M, Suh H, Leeper TC, Yang F, Mutschler H, Buratowski S, Meinhart A, Varani G
Nat Struct Mol Biol 17 1195-201 (2010)
PMID: 20818393

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137362 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Regulator of Ty1 transposition protein 103 Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 16.57 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q05543 (Residues: 1-131; Coverage: 32%)
Gene names: RTT103, YDR289C
Sequence domains: CID domain
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
DNA-directed RNA polymerase II subunit RPB1 Chain: B
Molecule details ›
Chain: B
Length: 14 amino acids
Theoretical weight: 1.54 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
UniProt:
  • Canonical: P04050 (Residues: 1675-1688; Coverage: 1%)
Gene names: D2150, RPB1, RPB220, RPO21, SUA8, YDL140C
Sequence domains: RNA polymerase Rpb1 C-terminal repeat

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

30 review citations

The RNA polymerase II CTD coordinates transcription and RNA processing.
Hsin et al. (2012)
29 more

5 mentions without citation

What's all the phos about? Insights into the phosphorylation state of the RNA polymerase II C-terminal domain via mass spectrometry.
LeBlanc et al. (2021)
4 more