› 2l2l

Solution NMR

Solution structure of the coiled-coil complex between MBD2 and p66alpha

Released:
DOI: 10.2210/pdb2l2l/pdb
PDB entry 2l2l coloured by chain, ensemble of 20 models, front view.
PDB entry 2l2l coloured by chain, ensemble of 20 models, side view.
PDB entry 2l2l coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
p66Alpha-MBD2 coiled-coil interaction and recruitment of Mi-2 are critical for globin gene silencing by the MBD2-NuRD complex.
Gnanapragasam MN, Scarsdale JN, Amaya ML, Webb HD, Desai MA, Walavalkar NM, Wang SZ, Zu Zhu S, Ginder GD, Williams DC
Proc Natl Acad Sci U S A 108 7487-92 (2011)
PMID: 21490301

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183274 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional repressor p66-alpha Chain: A
Molecule details ›
Chain: A
Length: 43 amino acids
Theoretical weight: 5.12 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q86YP4 (Residues: 137-178; Coverage: 7%)
Gene name: GATAD2A
Sequence domains: Coiled-coil and interaction region of P66A and P66B with MBD2
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Methyl-CpG-binding domain protein 2 Chain: B
Molecule details ›
Chain: B
Length: 36 amino acids
Theoretical weight: 4.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UBB5 (Residues: 360-393; Coverage: 8%)
Gene name: MBD2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Methyl-CpG-binding domain proteins: readers of the epigenome.
Du et al. (2015)
16 more

13 mentions without citation

The NuRD architecture.
Allen et al. (2013)
12 more