› 2l5a

Solution NMR

Structural basis for recognition of centromere specific histone H3 variant by nonhistone Scm3

Released:
DOI: 10.2210/pdb2l5a/pdb
PDB entry 2l5a coloured by chain, ensemble of 20 models, front view.
PDB entry 2l5a coloured by chain, ensemble of 20 models, side view.
PDB entry 2l5a coloured by chain, ensemble of 20 models, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structural basis for recognition of centromere histone variant CenH3 by the chaperone Scm3.
Zhou Z, Feng H, Zhou BR, Ghirlando R, Hu K, Zwolak A, Miller Jenkins LM, Xiao H, Tjandra N, Wu C, Bai Y
Nature 472 234-7 (2011)
PMID: 21412236

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135453 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone H3-like centromeric protein CSE4; Histone H4; Protein SCM3 Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 27.03 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P36012 (Residues: 151-228; Coverage: 34%)
  • Canonical: Q12334 (Residues: 93-172; Coverage: 36%)
  • Canonical: P02309 (Residues: 42-103; Coverage: 60%)
Gene names: CSE4, CSL2, D2155, HHF1, HHF2, N2752, SCM3, YBR009C, YBR0122, YDL139C, YKL049C, YKL262, YNL030W
Sequence domains:
Structure domains: Histone, subunit A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DGSA-distance geometry simulated annealing, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Histone chaperones in nucleosome assembly and human disease.
Burgess et al. (2013)
24 more

2 mentions without citation

Structure, assembly and reading of centromeric chromatin.
Maddox et al. (2012)
1 more