› 2l5d

Solution NMR

Solution Structures of human PIWI-like 1 PAZ domain with ssRNA (5'-pUGACA)

Released:
DOI: 10.2210/pdb2l5d/pdb
PDB entry 2l5d coloured by chain, ensemble of 20 models, front view.
PDB entry 2l5d coloured by chain, ensemble of 20 models, side view.
PDB entry 2l5d coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into piRNA recognition by the human PIWI-like 1 PAZ domain.
Zeng L, Zhang Q, Yan K, Zhou MM
Proteins 79 2004-9 (2011)
PMID: 21465557

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188564 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Piwi-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 134 amino acids
Theoretical weight: 15.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96J94 (Residues: 266-399; Coverage: 16%)
Gene names: HIWI, PIWIL1
Sequence domains: PAZ domain
Structure domains: paz domain
5'-R(*UP*GP*AP*CP*A)-3' Chain: B
Molecule details ›
Chain: B
Length: 5 nucleotides
Theoretical weight: 1.57 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DGSA-distance geometry simulated annealing, torsion angle dynamics
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

PIWI-interacting RNAs: small RNAs with big functions.
Ozata et al. (2019)
2 more

3 mentions without citation

Structural basis for sequence specific DNA binding and protein dimerization of HOXA13.
Zhang et al. (2011)
2 more