› 2l5u

Solution NMR

Structure of the first PHD finger (PHD1) from CHD4 (Mi2b)

Released:
DOI: 10.2210/pdb2l5u/pdb
PDB entry 2l5u coloured by chain, ensemble of 20 models, front view.
PDB entry 2l5u coloured by chain, ensemble of 20 models, side view.
PDB entry 2l5u coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Plant homeodomain (PHD) fingers of CHD4 are histone H3-binding modules with preference for unmodified H3K4 and methylated H3K9.
Mansfield RE, Musselman CA, Kwan AH, Oliver SS, Garske AL, Davrazou F, Denu JM, Kutateladze TG, Mackay JP
J Biol Chem 286 11779-91 (2011)
PMID: 21278251

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172109 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chromodomain-helicase-DNA-binding protein 4 Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14839 (Residues: 365-420; Coverage: 3%)
Gene name: CHD4
Sequence domains: PHD-finger
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Perceiving the epigenetic landscape through histone readers.
Musselman et al. (2012)
34 more

8 mentions without citation

Bivalent recognition of nucleosomes by the tandem PHD fingers of the CHD4 ATPase is required for CHD4-mediated repression.
Musselman et al. (2012)
7 more