› 2l6u

Solution NMR

Solution NMR Structure of Med25(391-543) Comprising the Activator-Interacting Domain (ACID) of Human Mediator Subuniti 25. Northeast Structural Genomics Consortium Target HR6188A

Released:
DOI: 10.2210/pdb2l6u/pdb
PDB entry 2l6u coloured by chain, ensemble of 20 models, front view.
PDB entry 2l6u coloured by chain, ensemble of 20 models, side view.
PDB entry 2l6u coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution NMR structure of MED25(391-543) comprising the activator-interacting domain (ACID) of human mediator subunit 25.
Eletsky A, Ruyechan WT, Xiao R, Acton TB, Montelione GT, Szyperski T
J Struct Funct Genomics 12 159-66 (2011)
PMID: 21785987

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mediator of RNA polymerase II transcription subunit 25 Chain: A
Molecule details ›
Chain: A
Length: 163 amino acids
Theoretical weight: 18.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q71SY5 (Residues: 391-543; Coverage: 21%)
Gene names: ACID1, ARC92, MED25, PTOV2, TCBAP0758
Sequence domains: Mediator complex subunit 25 PTOV activation and synapsin 2
Structure domains: Mediator complex subunit 25, ACID domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 95%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

A structural perspective on Mediator function.
Larivière et al. (2012)
2 more

3 mentions without citation

Twenty years of Mediator complex structural studies.
Verger et al. (2019)
2 more