› 2lax

Solution NMR

Structure of first WW domain of human YAP in complex with a human Smad1 doubly-phosphorilated derived peptide.

Released:
DOI: 10.2210/pdb2lax/pdb
PDB entry 2lax coloured by chain, ensemble of 20 models, front view.
PDB entry 2lax coloured by chain, ensemble of 20 models, side view.
PDB entry 2lax coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
A Smad action turnover switch operated by WW domain readers of a phosphoserine code.
Aragón E, Goerner N, Zaromytidou AI, Xi Q, Escobedo A, Massagué J, Macias MJ
Genes Dev 25 1275-88 (2011)
PMID: 21685363

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155555 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcriptional coactivator YAP1 Chain: A
Molecule details ›
Chain: A
Length: 40 amino acids
Theoretical weight: 4.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46937 (Residues: 170-205; Coverage: 7%)
Gene names: YAP1, YAP65
Sequence domains: WW domain
Mothers against decapentaplegic homolog 1 Chain: B
Molecule details ›
Chain: B
Length: 9 amino acids
Theoretical weight: 1.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15797 (Residues: 201-209; Coverage: 2%)
Gene names: BSP1, MADH1, MADR1, SMAD1

Ligands and Environments

No bound ligands
2 modified residues:
Ligand TPO 1 x TPO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 45%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

62 review citations

Molecular mechanisms of epithelial-mesenchymal transition.
Lamouille et al. (2014)
61 more

1 mention without citation

Structural determinants of Smad function in TGF-β signaling.
Macias et al. (2015)