› 2lc3

Solution NMR

Solution NMR structure of a helical bundle domain from human E3 ligase HECTD1. Northeast structural genomics consortium (NESG) target HT6305A

Released:
DOI: 10.2210/pdb2lc3/pdb
PDB entry 2lc3 coloured by chain, ensemble of 20 models, front view.
PDB entry 2lc3 coloured by chain, ensemble of 20 models, side view.
PDB entry 2lc3 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Entry authors: Lemak A, Yee A, Houliston S, Garcia M, Arrowsmith C, Dhe-Paganon S, Montelione GT, Northeast Structural Genomics Consortium (NESG), Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193929 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase HECTD1 Chain: A
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 10.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ULT8 (Residues: 1879-1966; Coverage: 3%)
Gene names: HECTD1, KIAA1131
Sequence domains: Basic tilted helix bundle domain
Structure domains: Transcription Termination Factor Rho, Rna-binding Domain; Chain A, Domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 87%
Refinement method: restrained molecular dynamics
Expression system: Escherichia coli

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