› 2leh

Solution NMR

Solution structure of the core SMN-Gemin2 complex

Released:
DOI: 10.2210/pdb2leh/pdb
PDB entry 2leh coloured by chain, ensemble of 32 models, front view.
PDB entry 2leh coloured by chain, ensemble of 32 models, side view.
PDB entry 2leh coloured by chain, ensemble of 32 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the core SMN-Gemin2 complex.
Sarachan KL, Valentine KG, Gupta K, Moorman VR, Gledhill JM, Bernens M, Tommos C, Wand AJ, Van Duyne GD
Biochem J 445 361-70 (2012)
PMID: 22607171

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127186 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gem-associated protein 2 Chain: A
Molecule details ›
Chain: A
Length: 189 amino acids
Theoretical weight: 21.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14893 (Residues: 95-280; Coverage: 66%)
Gene names: GEMIN2, SIP1
Sequence domains: Survival motor neuron (SMN) interacting protein 1 (SIP1)
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
Survival motor neuron protein Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 2.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q16637 (Residues: 26-51; Coverage: 9%)
Gene names: SMN, SMN1, SMN2, SMNC, SMNT
Sequence domains: Survival Motor Neuron, Gemin2-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 85%
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Diverse role of survival motor neuron protein.
Singh et al. (2017)
4 more

3 mentions without citation

Solution structure of the core SMN-Gemin2 complex.
Sarachan et al. (2012)
2 more