› 2li8

Solution NMR

The solution structure of the Lin28-ZnF domains bound to AGGAGAU of pre-let-7 miRNA

Released:
DOI: 10.2210/pdb2li8/pdb
PDB entry 2li8 coloured by chain, ensemble of 20 models, front view.
PDB entry 2li8 coloured by chain, ensemble of 20 models, side view.
PDB entry 2li8 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of pre-let-7 miRNA recognition by the zinc knuckles of pluripotency factor Lin28.
Loughlin FE, Gebert LF, Towbin H, Brunschweiger A, Hall J, Allain FH
Nat Struct Mol Biol 19 84-9 (2011)
PMID: 22157959

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190708 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Protein lin-28 homolog A Chain: A
Molecule details ›
Chain: A
Length: 74 amino acids
Theoretical weight: 8.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9H9Z2 (Residues: 124-186; Coverage: 30%)
Gene names: CSDD1, LIN28, LIN28A, ZCCHC1
Sequence domains: Zinc knuckle
Structure domains: Zinc finger, CCHC-type
RNA (5'-R(*AP*GP*GP*AP*GP*AP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 7 nucleotides
Theoretical weight: 2.28 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 72%
Refinement method: simulated annealing
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

21 review citations

Regulation of microRNA biogenesis.
Ha et al. (2014)
20 more

8 mentions without citation

Engineering RNA-binding proteins for biology.
Chen et al. (2013)
7 more