› 2lkx

Solution NMR

NMR structure of the homeodomain of Pitx2 in complex with a TAATCC DNA binding site

Released:
DOI: 10.2210/pdb2lkx/pdb
PDB entry 2lkx coloured by chain, ensemble of 17 models, front view.
PDB entry 2lkx coloured by chain, ensemble of 17 models, side view.
PDB entry 2lkx coloured by chain, ensemble of 17 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the K50 class homeodomain PITX2 bound to DNA and implications for mutations that cause Rieger syndrome.
Chaney BA, Clark-Baldwin K, Dave V, Ma J, Rance M
Biochemistry 44 7497-511 (2005)
PMID: 15895993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115551 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pituitary homeobox 2 Chain: A
Molecule details ›
Chain: A
Length: 68 amino acids
Theoretical weight: 8.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99697 (Residues: 85-144; Coverage: 19%)
Gene names: ARP1, PITX2, RGS, RIEG, RIEG1
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
DNA (5'-D(*GP*CP*TP*CP*TP*AP*AP*TP*CP*CP*CP*CP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 13 nucleotides
Theoretical weight: 3.89 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*GP*GP*GP*GP*AP*TP*TP*AP*GP*AP*GP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 43%
Refinement method: molecular dynamics
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

4 review citations

Molecular and developmental mechanisms of anterior segment dysgenesis.
Sowden JC. (2007)
3 more

8 mentions without citation

Transcription factor family-specific DNA shape readout revealed by quantitative specificity models.
Yang et al. (2017)
7 more