› 2lv6

Hybrid

The complex between Ca-Calmodulin and skeletal muscle myosin light chain kinase from combination of NMR and aqueous and contrast-matched SAXS data

Released:
DOI: 10.2210/pdb2lv6/pdb
PDB entry 2lv6 coloured by chain, front view.
PDB entry 2lv6 coloured by chain, side view.
PDB entry 2lv6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Contrast-matched small-angle X-ray scattering from a heavy-atom-labeled protein in structure determination: application to a lead-substituted calmodulin-peptide complex.
Grishaev A, Anthis NJ, Clore GM
J Am Chem Soc 134 14686-9 (2012)
PMID: 22908850

Function and Biology Details

Reaction catalysed: 
ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143960 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Calmodulin-1 Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.68 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 2-149; Coverage: 99%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair
Myosin light chain kinase 2, skeletal/cardiac muscle Chain: B
Molecule details ›
Chain: B
Length: 26 amino acids
Theoretical weight: 2.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9H1R3 (Residues: 566-591; Coverage: 4%)
Gene name: MYLK2

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Experimental Method: Solution NMR, X-ray solution scattering
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

8 review citations

Super-resolution in solution X-ray scattering and its applications to structural systems biology.
Rambo et al. (2013)
7 more

6 mentions without citation

Contrast-matched small-angle X-ray scattering from a heavy-atom-labeled protein in structure determination: application to a lead-substituted calmodulin-peptide complex.
Grishaev et al. (2012)
5 more