› 2lwe

Solution NMR

Solution structure of mutant (T170E) second CARD of human RIG-I

Released:
DOI: 10.2210/pdb2lwe/pdb
PDB entry 2lwe coloured by chain, ensemble of 20 models, front view.
PDB entry 2lwe coloured by chain, ensemble of 20 models, side view.
PDB entry 2lwe coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structure and dynamics of the second CARD of human RIG-I provide mechanistic insights into regulation of RIG-I activation.
Ferrage F, Dutta K, Nistal-Villán E, Patel JR, Sánchez-Aparicio MT, De Ioannes P, Buku A, Aseguinolaza GG, García-Sastre A, Aggarwal AK
Structure 20 2048-61 (2012)
PMID: 23063562

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Antiviral innate immune response receptor RIG-I Chain: A
Molecule details ›
Chain: A
Length: 100 amino acids
Theoretical weight: 11.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95786 (Residues: 95-190; Coverage: 10%)
Gene names: DDX58, RIGI
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: DGSA-distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Cytosolic sensing of viruses.
Goubau et al. (2013)
7 more

4 mentions without citation

Host DDX Helicases as Possible SARS-CoV-2 Proviral Factors: A Structural Overview of Their Hijacking Through Multiple Viral Proteins.
Squeglia et al. (2020)
3 more