› 2lzg

Solution NMR

NMR Structure of Mdm2 (6-125) with Pip-1

Released:
DOI: 10.2210/pdb2lzg/pdb
PDB entry 2lzg coloured by chain, ensemble of 5 models, front view.
PDB entry 2lzg coloured by chain, ensemble of 5 models, side view.
PDB entry 2lzg coloured by chain, ensemble of 5 models, top view.
Source organism: Homo sapiens
Primary publication:
Ordering of the N-terminus of human MDM2 by small molecule inhibitors.
Michelsen K, Jordan JB, Lewis J, Long AM, Yang E, Rew Y, Zhou J, Yakowec P, Schnier PD, Huang X, Poppe L
J Am Chem Soc 134 17059-67 (2012)
PMID: 22991965

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169499 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase Mdm2 Chain: A
Molecule details ›
Chain: A
Length: 125 amino acids
Theoretical weight: 14.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q00987 (Residues: 1-125; Coverage: 26%)
Gene name: MDM2
Sequence domains: SWIB/MDM2 domain
Structure domains: SWIB/MDM2 domain

Ligands and Environments

1 bound ligand:
Ligand 13Q 1 x 13Q
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 68%
Refinement method: torsion angle dynamics, DGSA-distance geometry simulated annealing
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Drugging the p53 pathway: understanding the route to clinical efficacy.
Khoo et al. (2014)
7 more

8 mentions without citation

Small-molecule inhibitors of the MDM2-p53 protein-protein interaction (MDM2 Inhibitors) in clinical trials for cancer treatment.
Zhao et al. (2015)
7 more