› 2m10

Solution NMR

trans form of a photoswitchable PDZ domain crosslinked with an azobenzene derivative

Released:
DOI: 10.2210/pdb2m10/pdb
PDB entry 2m10 coloured by chain, ensemble of 20 models, front view.
PDB entry 2m10 coloured by chain, ensemble of 20 models, side view.
PDB entry 2m10 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Kinetic response of a photoperturbed allosteric protein.
Buchli B, Waldauer SA, Walser R, Donten ML, Pfister R, Blöchliger N, Steiner S, Caflisch A, Zerbe O, Hamm P
Proc Natl Acad Sci U S A 110 11725-30 (2013)
PMID: 23818626

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171438 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 13 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q12923 (Residues: 1361-1456; Coverage: 4%)
Gene names: PNP1, PTP1E, PTPL1, PTPN13
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand 33B 1 x 33B
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 89%
Refinement method: torsion angle dynamics, molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Allostery in Its Many Disguises: From Theory to Applications.
Wodak et al. (2019)
8 more

4 mentions without citation

Implications of short time scale dynamics on long time processes.
El Hage et al. (2017)
3 more