› 2m1d

Solution NMR

Biosynthetic engineered B28K-B29P human insulin monomer structure in in water/acetonitrile solutions.

Released:
DOI: 10.2210/pdb2m1d/pdb
PDB entry 2m1d coloured by chain, ensemble of 20 models, front view.
PDB entry 2m1d coloured by chain, ensemble of 20 models, side view.
PDB entry 2m1d coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Biosynthetic engineered B28(K)-B29(P) human insulin monomer structure in water and in water/acetonitrile solutions.
Borowicz P, Bocian W, Sitkowski J, Bednarek E, Mikiewicz-Syguła D, Kurzynoga D, Stadnik D, Surmacz-Chwedoruk W, Koźmiński W, Kozerski L
J Biomol NMR 55 303-9 (2013)
PMID: 23404086

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-134182 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 47%
Refinement method: torsion angle dynamics, DGSA-distance geometry simulated annealing
Expression system: Not provided

Quick links

Citations

2 citation in other articles

Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
Bednarek et al. (2017)
1 more

1 mention without citation

Progress in Simulation Studies of Insulin Structure and Function.
Gorai et al. (2022)