Function and Biology Details
Reaction catalysed:
Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored, cell-surface proteins to 'shed' the extracellular domains.
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160275 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Disintegrin and metalloproteinase domain-containing protein 17
Molecule details ›
Chain: A
Length: 88 amino acids
Theoretical weight: 9.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: Membrane-proximal domain, switch, for ADAM17
Structure domains: Echistatin
Length: 88 amino acids
Theoretical weight: 9.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P78536 (Residues: 581-642; Coverage: 8%)
Sequence domains: Membrane-proximal domain, switch, for ADAM17
Structure domains: Echistatin
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
52%
Refinement method:
simulated annealing, torsion angle dynamics
Expression system: Escherichia coli
