Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153346 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4
Molecule details ›
Chain: W
Length: 43 amino acids
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Length: 43 amino acids
Theoretical weight: 4.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
- Canonical:
P46934 (Residues: 838-877; Coverage: 3%)
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Amiloride-sensitive sodium channel subunit alpha
Molecule details ›
Chain: P
Length: 11 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
Length: 11 amino acids
Theoretical weight: 1.06 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
- Canonical: P37088 (Residues: 638-648; Coverage: 2%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Chemical shift assignment:
86%
Refinement method:
simulated annealing
Expression systems:
- Escherichia coli
- Not provided
