› 2m3s

Solution NMR

Calmodulin, i85l, f92e, h107i, l112r, a128t, m144r mutant

Released:
DOI: 10.2210/pdb2m3s/pdb
PDB entry 2m3s coloured by chain, ensemble of 20 models, front view.
PDB entry 2m3s coloured by chain, ensemble of 20 models, side view.
PDB entry 2m3s coloured by chain, ensemble of 20 models, top view.
Source organism: Gallus gallus
Primary publication:
A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.
Moroz OV, Moroz YS, Wu Y, Olsen AB, Cheng H, Mack KL, McLaughlin JM, Raymond EA, Zhezherya K, Roder H, Korendovych IV
Angew Chem Int Ed Engl 52 6246-9 (2013)
PMID: 23630096

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calmodulin Chain: A
Molecule details ›
Chain: A
Length: 151 amino acids
Theoretical weight: 17.11 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P62149 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CAM, RCJMB04_24e7
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 94%
Refinement method: simulated annealing, matrix relaxation
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Design of Catalytic Peptides and Proteins Through Rational and Combinatorial Approaches.
Maeda et al. (2016)
4 more

2 mentions without citation

A single mutation in a regulatory protein produces evolvable allosterically regulated catalyst of nonnatural reaction.
Moroz et al. (2013)
1 more