› 2m85

Solution NMR

PHD Domain from Human SHPRH

Released:
DOI: 10.2210/pdb2m85/pdb
PDB entry 2m85 coloured by chain, ensemble of 20 models, front view.
PDB entry 2m85 coloured by chain, ensemble of 20 models, side view.
PDB entry 2m85 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
PHD domain from human SHPRH.
Machado LE, Pustovalova Y, Kile AC, Pozhidaeva A, Cimprich KA, Almeida FC, Bezsonova I, Korzhnev DM
J Biomol NMR 56 393-9 (2013)
PMID: 23907177

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase SHPRH Chain: A
Molecule details ›
Chain: A
Length: 73 amino acids
Theoretical weight: 8.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q149N8 (Residues: 652-716; Coverage: 4%)
Gene names: KIAA2023, SHPRH
Structure domains: Zinc/RING finger domain, C3HC4 (zinc finger)

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 90%
Refinement method: distance geometry
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

SHPRH as a new player in ribosomal RNA transcription and its potential role in homeostasis of ribosomal DNA repeats.
Lee et al. (2018)
2 more

1 mention without citation

PHD domain from human SHPRH.
Machado et al. (2013)