› 2m8s

Solution NMR

NMR Structure of the Cytoplasmic Tail of the Membrane Form of Heparin-binding EGF-like Growth Factor (proHB-EGF-CT) Complexed with the Ubiquitin Homology Domain of Bcl-2-associated Athanogene 1 from Mus musculus (mBAG-1-UBH)

Released:
DOI: 10.2210/pdb2m8s/pdb
PDB entry 2m8s coloured by chain, ensemble of 18 models, front view.
PDB entry 2m8s coloured by chain, ensemble of 18 models, side view.
PDB entry 2m8s coloured by chain, ensemble of 18 models, top view.
Source organisms:
Primary publication:
Nuclear Magnetic Resonance Structure of the Cytoplasmic Tail of Heparin Binding EGF-like Growth Factor (proHB-EGF-CT) Complexed with the Ubiquitin Homology Domain of Bcl-2-Associated Athanogene 1 from Mus musculus (mBAG-1-UBH).
Hung KW, Huang HW, Cho CC, Chang SC, Yu C
Biochemistry 53 1935-46 (2014)
PMID: 24628338

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178811 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
BAG family molecular chaperone regulator 1 Chain: A
Molecule details ›
Chain: A
Length: 97 amino acids
Theoretical weight: 10.8 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60739 (Residues: 137-233; Coverage: 27%)
Gene name: Bag1
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Proheparin-binding EGF-like growth factor Chain: B
Molecule details ›
Chain: B
Length: 24 amino acids
Theoretical weight: 2.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q99075 (Residues: 185-208; Coverage: 13%)
Gene names: DTR, DTS, HBEGF, HEGFL

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 66%
Refinement method: simulated annealing
Expression system: Escherichia coli

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Citations

2 review citations

The nucleotide exchange factors of Hsp70 molecular chaperones.
Bracher et al. (2015)
1 more