› 2mej

Solution NMR

Solution Structure of the Complex Between BCL-xL and the p53 Core Domain determined with PRE restraints

Released:
DOI: 10.2210/pdb2mej/pdb
PDB entry 2mej coloured by chain, ensemble of 20 models, front view.
PDB entry 2mej coloured by chain, ensemble of 20 models, side view.
PDB entry 2mej coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The DNA-binding domain mediates both nuclear and cytosolic functions of p53.
Follis AV, Llambi F, Ou L, Baran K, Green DR, Kriwacki RW
Nat Struct Mol Biol 21 535-43 (2014)
PMID: 24814347

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138115 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Bcl-2-like protein 1 Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.67 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07817 (Residues: 1-209; Coverage: 90%)
Gene names: BCL2L, BCL2L1, BCLX
Sequence domains:
Structure domains: Blc2-like
Cellular tumor antigen p53 Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 24.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04637 (Residues: 96-312; Coverage: 55%)
Gene names: P53, TP53
Sequence domains: P53 DNA-binding domain
Structure domains: Immunoglobulin-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 20%
Refinement method: molecular dynamics
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

p53 in survival, death and metabolic health: a lifeguard with a licence to kill.
Kruiswijk et al. (2015)
14 more

10 mentions without citation

The Structural Biology of Bcl-xL.
Lee et al. (2019)
9 more