› 2mh0

Solution NMR

Solution NMR structure of the p300 Taz2:ETAD1 complex

Released:
DOI: 10.2210/pdb2mh0/pdb
PDB entry 2mh0 coloured by chain, ensemble of 20 models, front view.
PDB entry 2mh0 coloured by chain, ensemble of 20 models, side view.
PDB entry 2mh0 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Structural insights into TAZ2 domain-mediated CBP/p300 recruitment by transactivation domain 1 of the lymphopoietic transcription factor E2A.
Lochhead MR, Brown AD, Kirlin AC, Chitayat S, Munro K, Findlay JE, Baillie GS, LeBrun DP, Langelaan DN, Smith SP
J Biol Chem 295 4303-4315 (2020)
PMID: 32098872

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147537 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Transcription factor E2-alpha Chain: A
Molecule details ›
Chain: A
Length: 39 amino acids
Theoretical weight: 4.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P15923 (Residues: 1-37; Coverage: 6%)
Gene names: BHLHB21, E2A, ITF1, TCF3
Histone acetyltransferase p300 Chain: B
Molecule details ›
Chain: B
Length: 92 amino acids
Theoretical weight: 10.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09472 (Residues: 1723-1812; Coverage: 4%)
Gene names: EP300, P300
Sequence domains: TAZ zinc finger
Structure domains: TAZ domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: torsion angle dynamics
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Biophysical insights into glucose-dependent transcriptional regulation by PDX1.
Usher et al. (2022)
1 other article

3 mentions without citation

Role of Intrinsic Protein Disorder in the Function and Interactions of the Transcriptional Coactivators CREB-binding Protein (CBP) and p300.
Dyson et al. (2016)
2 more