› 2mh3

Solution NMR

NMR structure of the basic helix-loop-helix region of the transcriptional repressor HES-1

Released:
DOI: 10.2210/pdb2mh3/pdb
PDB entry 2mh3 coloured by chain, ensemble of 20 models, front view.
PDB entry 2mh3 coloured by chain, ensemble of 20 models, side view.
PDB entry 2mh3 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
The basic helix-loop-helix region of the transcriptional repressor hairy and enhancer of split 1 is preorganized to bind DNA.
Popovic M, Wienk H, Coglievina M, Boelens R, Pongor S, Pintar A
Proteins 82 537-45 (2014)
PMID: 24403087

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172011 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcription factor HES-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 70 amino acids
Theoretical weight: 8.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14469 (Residues: 27-95; Coverage: 25%)
Gene names: BHLHB39, HES1, HL, HRY
Sequence domains: Helix-loop-helix DNA-binding domain
Structure domains: Helix-loop-helix DNA-binding domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 41%
Refinement method: restrained molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Notch-mediated lateral inhibition is an evolutionarily conserved mechanism patterning the ectoderm in echinoids.
Erkenbrack EM. (2018)
1 more

3 mentions without citation

Homodimeric and Heterodimeric Interactions among Vertebrate Basic Helix-Loop-Helix Transcription Factors.
Torres-Machorro AL. (2021)
2 more