› 2mjv

Solution NMR

Solution structures of second bromodomain of Brd4 with di-acetylated Twist peptide

Released:
DOI: 10.2210/pdb2mjv/pdb
PDB entry 2mjv coloured by chain, ensemble of 20 models, front view.
PDB entry 2mjv coloured by chain, ensemble of 20 models, side view.
PDB entry 2mjv coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Disrupting the interaction of BRD4 with diacetylated Twist suppresses tumorigenesis in basal-like breast cancer.
Shi J, Wang Y, Zeng L, Wu Y, Deng J, Zhang Q, Lin Y, Li J, Kang T, Tao M, Rusinova E, Zhang G, Wang C, Zhu H, Yao J, Zeng YX, Evers BM, Zhou MM, Zhou BP
Cancer Cell 25 210-25 (2014)
PMID: 24525235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130133 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Twist-related protein 1 Chain: A
Molecule details ›
Chain: A
Length: 12 amino acids
Theoretical weight: 1.3 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15672 (Residues: 68-79; Coverage: 6%)
Gene names: BHLHA38, TWIST, TWIST1
Bromodomain-containing protein 4 Chain: B
Molecule details ›
Chain: B
Length: 128 amino acids
Theoretical weight: 14.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O60885 (Residues: 333-460; Coverage: 9%)
Gene names: BRD4, HUNK1
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
1 modified residue:
Ligand ALY 2 x ALY

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 78%
Refinement method: simulated annealing, DGSA-distance geometry simulated annealing
Expression system: Not provided

Quick links

Citations

96 review citations

The mechanisms behind the therapeutic activity of BET bromodomain inhibition.
Shi et al. (2014)
95 more

1 mention without citation

Disrupting the interaction of BRD4 with diacetylated Twist suppresses tumorigenesis in basal-like breast cancer.
Shi et al. (2014)