› 2mls

Solution NMR

Membrane Bilayer complex with Matrix Metalloproteinase-12 at its Beta-face

Released:
DOI: 10.2210/pdb2mls/pdb
PDB entry 2mls coloured by chain, ensemble of 14 models, front view.
PDB entry 2mls coloured by chain, ensemble of 14 models, side view.
PDB entry 2mls coloured by chain, ensemble of 14 models, top view.
Source organism: Homo sapiens
Primary publication:
Ambidextrous binding of cell and membrane bilayers by soluble matrix metalloproteinase-12.
Koppisetti RK, Fulcher YG, Jurkevich A, Prior SH, Xu J, Lenoir M, Overduin M, Van Doren SR
Nat Commun 5 5552 (2014)
PMID: 25412686

Function and Biology Details

Reaction catalysed: 
Hydrolysis of soluble and insoluble elastin (1). Specific cleavages are also produced at -Ala(14)-|-Leu- and -Tyr(16)-|-Leu- in the B chain of insulin (2).
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153971 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Macrophage metalloelastase Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P39900 (Residues: 100-263; Coverage: 36%)
Gene names: HME, MMP12
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
Ligand PX4 125 x PX4
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 83%
Refinement method: molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Therapeutic Potential of Matrix Metalloproteinase Inhibition in Breast Cancer.
Radisky et al. (2017)
1 more

6 mentions without citation

Peripheral membrane associations of matrix metalloproteinases.
Van Doren et al. (2017)
5 more