› 2msr

Solution NMR

Solution structure of LEDGF/p75 IBD in complex with MLL1 peptide (140-160)

Released:
DOI: 10.2210/pdb2msr/pdb
PDB entry 2msr coloured by chain, ensemble of 20 models, front view.
PDB entry 2msr coloured by chain, ensemble of 20 models, side view.
PDB entry 2msr coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Validation and structural characterization of the LEDGF/p75-MLL interface as a new target for the treatment of MLL-dependent leukemia.
Cermáková K, Tesina P, Demeulemeester J, El Ashkar S, Méreau H, Schwaller J, Rezáčová P, Veverka V, De Rijck J
Cancer Res 74 5139-51 (2014)
PMID: 25082813

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + a [histone H3]-L-lysine(4) = S-adenosyl-L-homocysteine + a [histone H3]-N(6)-methyl-L-lysine(4)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130987 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
MLL cleavage product N320 Chain: A
Molecule details ›
Chain: A
Length: 21 amino acids
Theoretical weight: 2.27 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03164 (Residues: 140-160; Coverage: 1%)
Gene names: ALL1, CXXC7, HRX, HTRX, KMT2A, MLL, MLL1, TRX1
PC4 and SFRS1-interacting protein Chain: B
Molecule details ›
Chain: B
Length: 88 amino acids
Theoretical weight: 10.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75475 (Residues: 344-426; Coverage: 16%)
Gene names: DFS70, LEDGF, PSIP1, PSIP2
Sequence domains: Lens epithelium-derived growth factor (LEDGF)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 88%
Refinement method: torsion angle dynamics, molecular dynamics
Expression system: Escherichia coli

Quick links

Citations

13 review citations

Epigenetic modifications of histones in cancer.
Zhao et al. (2019)
12 more

2 mentions without citation

Structure, function and inhibition of critical protein-protein interactions involving mixed lineage leukemia 1 and its fusion oncoproteins.
Li et al. (2021)
1 more